quanti-blue solution  (InvivoGen)

Journal: The Cell Surface

Article Title: Acetylation and accessibility of Cryptococcus neoformans cell wall chitosans influence the strength of host immune responses

doi: 10.1016/j.tcsw.2026.100175

Figure Lengend Snippet: Responses of NF-κB-SEAP macrophage reporter cells expressing various PRRs to different Cn cells. The SEAP reporter gene is induced by NF-κB activation, and SEAP activity is quantified by spectrophotometry at 630 nm using Quanti-Blue as the substrate. WT Cn cultivated in YPD was compared to ( a ) mutant strains grown under the same conditions or ( b ) the same WT strain grown in YNB-U. Each strain and condition was tested in two independent experiments ( N = 2) with three replicates each ( n = 3). The whiskers show the range of data points within 1.5× the interquartile range. Statistical significance (* p < 0.01) was determined by one-way ANOVA with Tukey's multiple comparisons test between each sample and WT KN99 YPD. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: Following incubation, 20 μL of culture supernatant was collected to measure SEAP activity using Quanti-Blue (InvivoGen) as the substrate.

Techniques: Expressing, Activation Assay, Activity Assay, Spectrophotometry, Mutagenesis